Depletion of Free 30S Ribosomal Subunits in Escherichia coli by Expression of RNA Containing Shine-Dalgarno-Like Sequences
نویسندگان
چکیده
منابع مشابه
Depletion of free 30S ribosomal subunits in Escherichia coli by expression of RNA containing Shine-Dalgarno-like sequences.
We have constructed synthetic coding sequences for the expression of poly(alpha,L-glutamic acid) (PLGA) as fusion proteins with dihydrofolate reductase (DHFR) in Escherichia coli. These PLGA coding sequences use both GAA and GAG codons for glutamic acid and contain sequence elements (5'-GAGGAGG-3') that resemble the consensus Shine-Dalgarno (SD) sequence found at translation initiation sites in...
متن کاملCrystal structure of an RNA dodecamer containing the Escherichia coli Shine-Dalgarno sequence.
The synthetic dodecameric RNA fragment rUAAGGAGGUGAU resembles a region upstream of the initiation site in prokaryotic mRNAs whereas the pyrimidine-rich complementary strand is identical to the last 12 nucleotides of Escherichia coli 16 S rRNA. The complex thus serves as a model for the Shine-Dalgarno interaction which is required for proper initiation of translation. The crystal structure of r...
متن کاملEvaluating Representations for the Shine-Dalgarno Site in Escherichia coli
Several methods for identifying individual motif instance by exhaustive evaluation of k-mers (k ≤ 10) are applied to the pooled Upstream Regions (USR) of all 4289 Escherichia coli ORFs. Instances of the Shine-Dalgarno (SD) site are readily identified using these methods. Using these motif instances as starting points, various motif representations and training methods, including several new alg...
متن کاملSelection of Shine-Dalgarno sequences in plastids
Like bacterial genes, most plastid (chloroplast) genes are arranged in operons and transcribed as polycistronic mRNAs. Plastid protein biosynthesis occurs on bacterial-type 70S ribosomes and translation initiation of many (but not all) mRNAs is mediated by Shine-Dalgarno (SD) sequences. To study the mechanisms of SD sequence recognition, we have analyzed translation initiation from mRNAs contai...
متن کاملDepletion of Shine-Dalgarno Sequences Within Bacterial Coding Regions Is Expression Dependent
Efficient and accurate protein synthesis is crucial for organismal survival in competitive environments. Translation efficiency-the number of proteins translated from a single mRNA in a given time period-is the combined result of differential translation initiation, elongation, and termination rates. Previous research identified the Shine-Dalgarno (SD) sequence as a modulator of translation ini...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2002
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.184.2.494-502.2002